Table 1.
Binding measurements for wild-type T-cell receptors
| TCR | Peptide/MHC | kon (per m second) | koff (per second) | t1/2 (seconds) | KD (μm) | Activity | References |
|---|---|---|---|---|---|---|---|
| CT26 | AH1(A5)/Ld | 58 000 | 0·11 | 6·3 | 1·9 | Agonist | 3 |
| P14 | gp33/Db | 400 000 | 0·975 | 0·7 | 2·4 | Agonist | 4 |
| 2C | p2Ca/Ld | 8300 | 0·027 | 25·7 | 3·3 | Agonist | 5 |
| 2C | QL9/Ld | 6350 | 0·025 | 26·8 | 3·9 | Agonist | 5 |
| CT26 | AH1/Ld | 61 000 | 0·35 | 2·0 | 5·7 | Agonist | 3 |
| OT-1 | OVA/Kb | 3720 | 0·022 | 31·5 | 5·9 | Agonist | 6 |
| OT-1 | OVA(G4)/Kb | 900 | 0·009 | 77 | 10 | Weak agonist | 7 |
| CT26 | AH1(A7)/Ld | 16 000 | 0·28 | 2·4 | 18 | Weak agonist | 3 |
| 2C | SIY/Kb | 22 000 | 0·464 | 1·5 | 27·4 | Agonist | 8 |
| AHIII 12.2 | p1058/Db | 6610 | 0·538 | 1·2 | 81·4 | Weak agonist | 9 |
| 2C | dEV8/Kb | 2200 | 0·185 | 3·7 | 84·1 | Antagonist | 5 |
| B7 | Tax/HLA-A2 | 96 000 | 0·13 | 5·2 | 1·2 | Agonist | 10 |
| A6 | Tax/HLA-A2 | 49 000 | 0·11 | 6·1 | 1·9 | Agonist | 10 |
| G10 | HIVgagSLY/HLA-A2 | 330 000 | 0·06 | 11·2 | 2·2 | Agonist | 11 |
| GRB | Flu/HLA-B27 | 39 000 | 0·09 | 7·4 | 3 | 12 | |
| JM22 | Flu/HLA-A2 | 31 000 | 0·16 | 4·2 | 5·2 | 13 | |
| G10 | HIVgagSLF/HLA-A2 | 340 000 | 0·16 | 4·2 | 5·2 | Agonist | 11 |
| CMV | pp65/HLA-A2 | 70 000 | 0·44 | 1·5 | 6·3 | 14 | |
| gp100 | gp100/HLA-A2 | 31 000 | 0·23 | 2·9 | 7 | 12 | |
| AHIII 12.2 | p1049/HLA-A2 | 26 200 | 0·295 | 2·3 | 11·3 | Agonist | 9 |
| LC13 | FLR(A)/HLA-B8 | 35 800 | 0·42 | 1·7 | 12·5 | Agonist | 15 |
| AM3 | EBV/HLA-A24 | 7300 | 0·21 | 3·2 | 28 | 12 | |
| 1G4 | NY-ESO-1/HLA-A2 | 40 000 | 0·128 | 6·4 | 32 | Agonist | 16 |
| TEL | tel/HLA-A2 | 3500 | 0·14 | 4·8 | 40 | 12 | |
| LC13 | FLR(F)/HLA-B8 | 2620 | 0·35 | 2·0 | 132 | Antagonist | 15 |
| 172.10 | MBP1-11[4Y]/I-Au | 37 200 | 0·219 | 3·1 | 5·9 | Agonist | 17 |
| 3.L2 | Hb/I-Ek | 5557 | 0·06 | 10·8 | 12 | Agonist | 18 |
| 1934.4 | MBP1-11[4Y]/I-Au | 5130 | 0·16 | 4·2 | 31 | Weak agonist | 17 |
| 2B4 | MCC/I-Ek | 633 | 0·057 | 11·7 | 90 | Agonist | 2 |
| MAW 13 | M-HSP/HLA-DR3 | 4000 | 0·12 | 5·6 | 30 | 12 | |
| AH1.23 | C-HSP/HLA-DR4 | 4400 | 0·16 | 4·2 | 36 | 12 | |
| 1A12 | MBP/HLA-DR2 | 2100 | 0·17 | 3·9 | 81 | 12 | |
| 2E11 | MBP/HLA-DR2 | 5900 | 0·73 | 0·9 | 123 | 12 |
KD, equilibrium binding constant; kon, association rate; koff, dissociation rate; MHC, major histocompatibility complex; t1/2, half-life of the interaction; TCR, T-cell receptor.
Measurements performed at 25°.