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Interleukin-15 (IL-15) is not essential for macrophage (MΦ) differentiation but strongly induces MΦ activation in vivo. MΦ were freshly isolated from wild-type IL-15+/+, IL-15−/− and IL-15tg lung (a) and peritoneal cavity (b). The expression of costimulatory molecules and major histocompatibility complex (MHC) class II was analysed by fluorescence-activated cell sorter staining on F4/80+ gated MΦ; (n = 4 mice/group). Significance was calculated using Student's t-test.
