Table 1.

Oligonucleotides used for reverse transcriptase–polymerase chain reaction (RT-PCR) analysis

Gene	Forward	Reverse	T (°)
IL-7Rα	AGCTGTTTCTGGAGAAAGTGG	AACGACTTTCAGGTCAGAGGG	61·5
VpreB	CGTCTGTCCTGCTCATGCT	ACGGCACAGTAATACACAGCC	53·8
EBF	CAAGACAAGAACCCTGAAATG	GTAACCTCTGGAAGCCGTAGT	55·5
E2A	CATCCATGTCCTGCGAAGCCAC	TTCTTGTCCTCTTCGGCGTCGG	65
Pax5	GTCATCGGTGAGCACCGACTC	GAAGCCATGGCTGAATACTC	58·7
Lysozyme M	GTCAGCCTGGCCGACTGGGTG	GACTCCGCAGTTCCGAATATA	65
M-CSFR	GCGATGTGTGAGCAATGGCAGT	AGACCGTTTTGCGTAAGACCTG	61·5
PU.1	CGGATGACTTGGTTACTTACG	GTAGGAAACCTGGTGACTGAG	55·5
F4/80	CTTTGGCTATGGGCTTCCAGTC	GCAACGAGGACAGAGTTTATCGTG	60
G-CSFR	CTCAAACCTATCCTGCCTCATG	TCCAGGCAGAGATGAGCGAATG	55·5
VH11	GCAATAAACTACGCACCATCCA	TTCGCACATTGTCCTCCGA	58
VH12	CTTCTACAACCCATCCCTCCAG	TACATGGCTGTGTCCTCTGTGG	58
GADPH	CGCCTGGTCACCAGGGCTGC	CACCACCCTGTTGCTGTAGCC	55

EBF, early B-cell factor; E2A, E box protein; GADPH, glyceraldehyde 3-phosphate dehydrogenase; G-CSFR, granulocyte colony-stimulating factor receptor; IL, interleukin; IL-7Rα, alpha subunit for IL-7 receptor; M-CSFR, macrophage colony-stimulating factor receptor; Pax5, paired box 5; VH, variable heavy chain 11 or 12; VpreB, surrogate light chain.