Table 1.
Antibodies used
| Specificity | Clone | Isotype | Conjugate | Dilution | Supplier |
|---|---|---|---|---|---|
| CD6 | Ox 52 | IgG2a, κ | Unconjugated | 1 : 2000 | Serotec1 |
| T lymphocytes (α/β) | R73 | IgG1 | Unconjugated | 1 : 20 | Serotec |
| MHC class II | Ox 6 | IgG1 | Biotinylated | 1 : 50 | Serotec |
| CD4 | W3/25 | IgG1 | Biotinylated FITC APC | 1 : 100 1 : 1000 1 : 250 | Serotec |
| CD25 | Ox 39 | IgG1 | Biotinylated PE | 1 : 50 1 : 50 | Serotec |
| Neuropilin 1 (Nrp1) | 130604 | IgG1 | Unconjugated | 1 : 100 | RD Systems2 |
| Isotope control for Nrp1 | IgG1 | Unconjugated | 1 : 100 | RD Systems3 | |
| Foxp3 | FJK-16s | IgG2a, κ | FITC | 1 : 10 | BioLegend4 |
| Isotype control for Foxp3 (mAB) | IgG2a | FITC | 1 : 10 | BioLegend | |
| B cells [F (ab) fragment of rat IgG] | Ox 12 | IgG1 | Unconjugated | 1 : 1000 | Serotec |
| Monocytes, macrophages, DCs, granulocytes | ED9 | IgG1 | Unconjugated | 1 : 500 | Serotec |
| NK cells | CD161 | 100/78 | Unconjugated | 1 : 500 | Serotec |
1
Kidlington, Oxford, UK;
2
Abington, Oxford, UK;
3
Wiesbdaen, Germany;
4
San Diego, CA.
DC, dendritic cell; IgG, immunoglobulin G; mAb, monoclonal antibody; MHC, major histocompatibility complex; NK, natural killer.