Fig. 3.

Increased rod number and fewer UV cones in lor^p25bbtl and fby mutants. (A–C) Confocal images of eyes from 5-dpf WT (A), lor^p25bbtl (B), and fby (C) larvae, immunolabeled for UV opsin (green) and rods (red). Eyes from lor^p25bbtl and fby mutant larvae demonstrate a dramatic deficit in UV cones, with fby displaying a more severe phenotype; in this particular sample, no UV cones were detected. (D) Graph showing the average number of rods (red) and UV cones (green) per unit area quantified from confocal images of lor^p25bbtl mutant and WT retinas (mean + SD). (E and F) Expression analysis of opsins (E) and arrestins (F) by RT-PCR from RNA extracted from 5-dpf WT and lor^p25bbtl larvae. The RT-PCR product for UV opsin was reduced and products for rod opsin and rod arrestin were increased in lor^p25bbtl (red boxes).