Effect of AF3 on voltage-activated inward currents. (A) Voltage-gated transient-inward currents recorded before and 9 min after AF3 application. Note the increase in the amplitude of the Ipeak and the Iss current. Recordings were made in calcium-Ringer solution with cesium acetate in recording micropipettes. (B) Ipeak current–voltage relationship for the experiment shown in A. Control:
AF3 (1 μM):
Post-wash:
(C) Iss current–voltage relationship for the experiment shown in A. Control:
AF3 (1 μM):
Post-wash:
(D) Potentiation of the Ipeak current after AF3 (1 μM) application (paired t-test, n = 9, **P ≤ 0.01, *P ≤ 0.05). (E) Long lasting potentiation of the Iss current after AF3 application (paired t-test, n = 9, **P ≤ 0.01, *P ≤ 0.05).