Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1984 Aug;45(2):505–510. doi: 10.1128/iai.45.2.505-510.1984

Longitudinal study on the in vitro immune response to Plasmodium falciparum in Sudan.

J A Vande Waa, J B Jensen, M A Akood, R Bayoumi
PMCID: PMC263274  PMID: 6378799

Abstract

Inhibition of Plasmodium falciparum in vitro by human immune serum provides needed information in understanding antimalarial immune mechanisms. Longitudinal, dry season-to-wet season changes in antimalarial activities were studied in sera isolated from 62 individuals living in an area of hyperendemic but unstable malaria. Highly synchronous cultures of P. falciparum were used to distinguish and quantitate two antimalarial activities, merozoite invasion inhibition, and intraerythrocytic parasite retardation. In 54% of the individuals, intraerythrocytic parasite retardation activity increased significantly, nearly threefold, in wet-season sera as compared with dry-season sera. Merozoite invasion inhibition activity was moderate and did not change seasonally. Merozoite invasion inhibition was, however, correlated to parasite-specific immunoglobulin G titers and total serum immunoglobulin G concentrations. These results confirm earlier studies which demonstrate two antimalarial activities in Sudanese sera and provide evidence that intraerythrocytic parasite retardation activity plays a role in antimalarial immunity.

Full text

PDF
509

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison A. C., Eugui E. M. The role of cell-mediated immune responses in resistance to malaria, with special reference to oxidant stress. Annu Rev Immunol. 1983;1:361–392. doi: 10.1146/annurev.iy.01.040183.002045. [DOI] [PubMed] [Google Scholar]
  2. COHEN S., McGREGOR I. A., CARRINGTON S. Gamma-globulin and acquired immunity to human malaria. Nature. 1961 Nov 25;192:733–737. doi: 10.1038/192733a0. [DOI] [PubMed] [Google Scholar]
  3. Clark I. A., Allison A. C., Cox F. E. Protection of mice against Babesia and Plasmodium with BCG. Nature. 1976 Jan 29;259(5541):309–311. doi: 10.1038/259309a0. [DOI] [PubMed] [Google Scholar]
  4. Clark I. A., Virelizier J. L., Carswell E. A., Wood P. R. Possible importance of macrophage-derived mediators in acute malaria. Infect Immun. 1981 Jun;32(3):1058–1066. doi: 10.1128/iai.32.3.1058-1066.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cohen S., Butcher G. A. Properties of protective malarial antibody. Immunology. 1970 Aug;19(2):369–383. [PMC free article] [PubMed] [Google Scholar]
  6. Deans J. A., Cohen S. Immunology of malaria. Annu Rev Microbiol. 1983;37:25–49. doi: 10.1146/annurev.mi.37.100183.000325. [DOI] [PubMed] [Google Scholar]
  7. Hall C. L., Haynes J. D., Chulay J. D., Diggs C. L. Cultured Plasmodium falciparum used as antigen in a malaria indirect fluorescent antibody test. Am J Trop Med Hyg. 1978 Sep;27(5):849–852. doi: 10.4269/ajtmh.1978.27.849. [DOI] [PubMed] [Google Scholar]
  8. Jensen J. B., Boland M. T., Akood M. Induction of crisis forms in cultured Plasmodium falciparum with human immune serum from Sudan. Science. 1982 Jun 11;216(4551):1230–1233. doi: 10.1126/science.7043736. [DOI] [PubMed] [Google Scholar]
  9. Jensen J. B., Boland M. T., Allan J. S., Carlin J. M., Vande Waa J. A., Divo A. A., Akood M. A. Association between human serum-induced crisis forms in cultured Plasmodium falciparum and clinical immunity to malaria in Sudan. Infect Immun. 1983 Sep;41(3):1302–1311. doi: 10.1128/iai.41.3.1302-1311.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jensen J. B., Boland M. T., Hayes M., Akood M. A. Plasmodium falciparum: rapid assay for in vitro inhibition due to human serum from residents of malarious areas. Exp Parasitol. 1982 Dec;54(3):416–424. doi: 10.1016/0014-4894(82)90051-0. [DOI] [PubMed] [Google Scholar]
  11. Jensen J. B. Concentration from continuous culture of erythrocytes infected with trophozoites and schizonts of Plasmodium falciparum. Am J Trop Med Hyg. 1978 Nov;27(6):1274–1276. doi: 10.4269/ajtmh.1978.27.1274. [DOI] [PubMed] [Google Scholar]
  12. Jensen J. B., Hoffman S. L., Boland M. T., Akood M. A., Laughlin L. W., Kurniawan L., Marwoto H. A. Comparison of immunity to malaria in Sudan and Indonesia: crisis-form versus merozoite-invasion inhibition. Proc Natl Acad Sci U S A. 1984 Feb;81(3):922–925. doi: 10.1073/pnas.81.3.922. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jensen J. B., Trager W. Plasmodium falciparum in culture: use of outdated erthrocytes and description of the candle jar method. J Parasitol. 1977 Oct;63(5):883–886. [PubMed] [Google Scholar]
  14. Lambros C., Vanderberg J. P. Synchronization of Plasmodium falciparum erythrocytic stages in culture. J Parasitol. 1979 Jun;65(3):418–420. [PubMed] [Google Scholar]
  15. MCGREGOR I. A., WILLIAMS K., VOLLER A., BILLEWICZ W. Z. IMMUNOFLUORESCENCE AND THE MEASUREMENT OF IMMUNE RESPONSE TO HYPERENDEMIC MALARIA. Trans R Soc Trop Med Hyg. 1965 Jul;59:395–414. doi: 10.1016/0035-9203(65)90057-x. [DOI] [PubMed] [Google Scholar]
  16. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  17. McGregor I. A., Rowe D. S., Wilson M. E., Billewicz W. Z. Plasma immunoglobulin concentrations in an African (Gambian) community in relation to season, malaria and other infections and pregnancy. Clin Exp Immunol. 1970 Jul;7(1):51–74. [PMC free article] [PubMed] [Google Scholar]
  18. Phillips R. S., Trigg P. I., Scott-Finnigan T. J., Bartholomew R. K. Culture of Plasmodium falciparum in vitro: a subculture technique used for demonstrating antiplasmodial activity in serum from some Gambians, resident in an endemic malarious area. Parasitology. 1972 Dec;65(3):525–535. doi: 10.1017/s0031182000044139. [DOI] [PubMed] [Google Scholar]
  19. Playfair J. H. Immunity to malaria. Br Med Bull. 1982 May;38(2):153–159. doi: 10.1093/oxfordjournals.bmb.a071752. [DOI] [PubMed] [Google Scholar]
  20. Taverne J., Dockrell H. M., Playfair J. H. Endotoxin-induced serum factor kills malarial parasites in vitro. Infect Immun. 1981 Jul;33(1):83–89. doi: 10.1128/iai.33.1.83-89.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Taylor D. W., Siddiqui W. A. Recent advances in malarial immunity. Annu Rev Med. 1982;33:69–96. doi: 10.1146/annurev.me.33.020182.000441. [DOI] [PubMed] [Google Scholar]
  22. Trager W., Jensen J. B. Human malaria parasites in continuous culture. Science. 1976 Aug 20;193(4254):673–675. doi: 10.1126/science.781840. [DOI] [PubMed] [Google Scholar]
  23. Wozencraft A. O., Dockrell H. M., Taverne J., Targett G. A., Playfair J. H. Killing of human malaria parasites by macrophage secretory products. Infect Immun. 1984 Feb;43(2):664–669. doi: 10.1128/iai.43.2.664-669.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES