Skip to main content
. 2009 Feb 5;4(2):e4341. doi: 10.1371/journal.pone.0004341

Figure 5. Targeting strategy for the conditional inactivation of the Cdh22 gene.

Figure 5

(A) Restriction maps of the mouse wild type Cdh22 locus, the targeting vector, and the targeted allele. Relevant restriction sites: E, EcoRI; K, KpnI. Vertical dotted lines highlight homologous regions. (B) Southern analysis of genomic DNA from ES cells (EcoRI digestion), with the 5′-probe illustrating the 21.5 kb wild type (wt) and 10.5 kb targeted (cond) alleles. (C) Southern analysis of genomic DNA from ES cells (EcoRI digestion), with the 3′ probe illustrating the 21.5 kb wild type (wt) and 8.9 kb targeted (cond) alleles.