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. 2009 Feb 5;4(2):e4352. doi: 10.1371/journal.pone.0004352

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) The Meg8 alleles contain an informative restriction site polymorphism in that a HgaI site is present in the 129S1 allele in comparison to CzechII/Ei. RT-PCR products with AF498299 1Up/294Dn primers for Meg8 from the four intraspecific crosses were digested with and without HgaI, verifying the imprinted expression of this gene. (B) Northern blots were performed on adult tissues (left) and embryos harvested at different time points (right). Meg8 levels were highest in brain and testis. In addition, Meg8 RNA increases as a functional of embryonic development.