Figure 5. Inhibition of the greenbug and human AChEs by AMTS7–AMTS20.
Extracts of greenbugs and human RBCs in 0.1 M sodium phosphate buffer (see Methods) were exposed to AMTS7–AMTS20 at 6.0 µM for one hour at room temperature. Radiometric assays were used to determine AChE activity in aliquots of treated samples either immediately (empty symbols) or after overnight dialysis with 2 changes of buffer in 100-fold excess (filled symbols). The difference between the paired measurements (i.e., the recovery after dialysis) indicates reversible inhibition.
