Figure 1.

Disruption of the Slc2a3 gene by gene trapping. (A) Localization of the gene trap between exons 1 and 2 of the Slc2a3 gene. (B) Integration of the gene trap vector in intron 1 of the Slc2a3 gene in ES cell clone XG611 results in a fusion transcript containing exon 1 and the β-geo cassette. With the indicated primer pairs, four specific RT-PCR products were detected. (C) Southern blot analysis confirmed the single integration of the gene trap vector into the Slc2a3 gene of clone XG611. Genomic DNA was digested with BglII or NcoI and analyzed by Southern blotting with the indicated probe. A single 7 and 5·5 kb band respectively was detected in each panel.