Figure 2. Isosurface rendering of heart morphogenesis in wild type and LZoep mutants.
cmlc2-GFP cells label the cardiac cone and heart tube. Imaris software created an isosurface representing points of constant fluorescent intensity within the 3D volume. Cardiac cone is initially symmetric in both the wild type (A, B) and the LZoep mutant (F, G). Note the leftwards and anterior displacement of the heart in wild type (C-E) but not in LZoep mutants (H-J). For detailed views from different angles see Supplemental Movies 2 (wild type) and 6 (LZoep). Pictures were cropped to keep the heart in the center and do not maintain the positions of the coordinate systems. For movements with respect to coordinate systems, see Supplemental Movies 2 (wild type) and 6 (LZoep).