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. Author manuscript; available in PMC: 2009 Jan 29.
Published in final edited form as: J Proteome Res. 2007 Aug 14;6(9):3780–3787. doi: 10.1021/pr070278r

Figure 4.

Figure 4

Comparison of 2DE- and 1DE-LC–MS approaches using MicroSol fraction F2 (pI 4.6-5.4) for quantitation of plasmodial proteins. The analysis was restricted to proteins matching a predefined stringent quantitative filter criterion (3 top matched unique peptides in a Mascot search using MuDPIT scoring) for protein identification. Although the 1DE-LC–MS approach offered a 3-fold expansion in the quantitative profiling capacity, only 18 of the total 107 proteins were identified using both techniques, making the two approaches complementary.