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. Author manuscript; available in PMC: 2009 Jan 29.
Published in final edited form as: J Proteome Res. 2007 Aug 14;6(9):3780–3787. doi: 10.1021/pr070278r

Figure 5.

Figure 5

Analysis of the HDP containing fraction F5 (pI 7-10) for P. falciparum SHMT informed by the predicted molecular weight and pI of the protein (49.7 kDa, pI 8.29). (A) Pixels (1 mm) from the 40-55 kDa region of the gel were analyzed as shown, with SHMT being routinely identified in slice 3. HDP, hemeoglobin-derived products. (B) Series of protocol optimizations that led to progressive improvements in the unique peptide sequence coverage of plasmodial SHMT from 7 to 45%.