Table 1.
Isoleucine Containing P. falciparum SHMT Peptides Selected for the Inclusion List Used in this Study
| peptide sequence | modification | mass addition (Da) | inclusion list m/z (expt)a |
|---|---|---|---|
| IAAVACQLK | carbamidomethyl (C) | + 57.92 | 972.54 (486.27++) |
| IAAVACQLK | carbamidomethyl (C) | + 57.92 | 979.59 (489.8++) |
| + heavy isoleucine | + 7.0 | ||
| NLDLVTNGTDNHLIVVDLR | none | - | 2120.17 (706.7+++) |
| NLDLVTNGTDNHLIVVDLR | heavy isoleucine | + 7.0 | 2127.17 (709.5+++) |
| AILLTDELQQK | none | - | 1270.71 (635.3++) |
| AILLTDELQQK | heavy isoleucine | + 7.0 | 1277.76 (638.8++) |
a
The predicted isoleucine-labeled masses were calculated as a mass addition of 7 Da (3.5++ m/z), and known post-translational modifications (e.g., carbamidomethylation/methionine oxidation) were accounted for as shown.