Table 2.
Quantitative Analysis of P. falciparum SHMT and Enolase in 50/50 Isoleucine Labeled Asynchronous Culturesa
| SHMT peptides | intensityb (unlabeled) | intensity (labeled) | intensity ratiod unlab/lab | areac (unlabeled) | area (labeled) | area ratiod unlab/lab |
|---|---|---|---|---|---|---|
| IAAVACQLK | 10420.5 | 7009.5 | 1.48 | 2729.5 | 1733.5 | 1.57 |
| NLDLVTNGTDNHLIVVDLR | 6654 | 6893 | 0.96 | 1503 | 1329.5 | 1.13 |
| AILLTDELK 1 | 34907 | 28738 | 1.21 | 11089.5 | 12494 | 0.88 |
| AILLTDELK2 | 16942 | 16790 | 1.01 | 4220.5 | 5779 | 0.73 |
| AILLTDELK3 | 46062 | 29551 | 1.55 | 13551 | 8702 | 1.55 |
| Av | 1.24 | 1.17 | ||||
| SD | 0.26 | 0.38 |
| Enolase peptides | intensityb (unlabeled) | intensity (labeled) | intensity ratiod unlab/lab | areac (unlabeled) | area (labeled) | area ratiod unlab/lab |
|---|---|---|---|---|---|---|
| IDNLMVEELDGSK | 43747 | 53119 | 0.82 | 14597 | 17842 | 0.81 |
| TGAQLVDLYIDLVK | 70645 | 78265 | 0.90 | 24175 | 22481 | 1.07 |
| IEESLGNNAVFAGEK | 53278 | 42321 | 1.25 | 14265 | 13405 | 1.06 |
| KIDNLMVEELDGSK | 109725 | 102055 | 1.07 | 35754 | 34561 | 1.03 |
| DVQIVGDDLLVTNPTR | 125742 | 116527 | 1.07 | 39532 | 37666 | 1.04 |
| IAMDVAASEFYNSENK | 417155 | 369467 | 1.12 | 136575 | 135000 | 1.01 |
| AAVPSGASTGIYEALELR | 338710 | 279393 | 1.21 | 114339 | 83727 | 1.36 |
| LSFQEFMIVPVGAPSFK | 106882 | 95358 | 1.12 | 35785 | 30549 | 1.17 |
| LIGMNCTEQK | 95768 | 79331 | 1.20 | 32917 | 22131 | 1.48 |
| YPIVSIEDPFDQDDWENYAK | 40256 | 38809 | 1.03 | 14380 | 10982 | 1.30 |
| Av | 1.08 | 1.13 | ||||
| SD | 0.13 | 0.19 |
a
Two quantitative parameters were considered in the experiments for each labeled and unlabeled peptide pair analysis of P. falciparum SHMT and enolase data.
b
Intensity: average peak heights of the isotopic envelope.
c
Area: summation of the area under the isotopic envelope.
d
The final ratio for each parameter was expressed as the ratio of unlabeled:labeled peptide.