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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Mol Cancer Res. 2009 Jan;7(1):99–107. doi: 10.1158/1541-7786.MCR-08-0309

FIGURE 4.

FIGURE 4

MAPK inhibitors protect cells against FDH-induced apoptosis. A. Effects of the ERK1/2 inhibitor PD98059 and the JNK inhibitor SP600125 on phosphorylation of MAPKs and their substrates in FDH-expressing cells. Concentrations of inhibitors (μM) are indicated. B. Apoptosis detected by FACS flow cytometry in FDH expressing PC-3 cells: in the absence of MAPK inhibitors (left panel); in the presence of 10 μM of the JNK inhibitor SP600125 (middle panel); in the presence of 30 μM the ERK1/2 inhibitor (right panel). Time of FDH post-transfection is indicated. C. Colony formation by PC-3 cells expressing FDH and treated with SP600125, PD98059 or both simultaneously.