Table 2.

The majority of 0.2μm microsphere⁺ DCs that localize within the draining LNs phenotype as CD11c⁺CD11b⁺, while the majority of cells containing 1.0μm microspheres phenotype as CD11c⁻CD11b⁺

Experimental groups^a	Phenotype of microsphere⁺ cells residing in the draining LN^b
Experimental groups^a	CD11c⁺CD11b⁺ (%)	CD11c⁻CD11b⁺ (%)
0.2μm microspheres	76.3±1.1	13.8±0.8
1.0μm microspheres	30.5±2.8^c	62.8±1.4^c

1.0μm or 0.2μm green fluorescent microspheres (50μl of a 0.25% solution) were injected into hind footpads of C3H/HeN mice (3 per group).

Single cell suspensions were prepared from the draining popliteal lymph nodes after 48 hours. Cells were stained with antibodies specific for CD11c and CD11b and analyzed by FACScan. Phenotype analysis was done on microsphere⁺ cells. At least 1000 microsphere⁺ events were analyzed.

Differences between the percentage of 0.2μm microsphere⁺ DCs and the percentage of 1.0μm microsphere⁺ DCs were significant (p<0.001-0.005)