Table 1. Number of vlsE sequences analyzed from different tissues and time points during experimental infection of immunocompetent C3H/HeN mice or SCID mice with B. burgdorferi B31.
Days post infection | No. of sequences | |||||||||
C3H/HeN | SCIDa | |||||||||
Bladder | Heart | Joint | Ear | Skin | Bladder | Heart | Joint | Ear | Skin | |
4 | NCb | NC | NC | NC | 43* | NC | NC | NC | NC | 23§* |
7 | 72 | 71 | 76 | NC | 6* | 4 | 15 | 20 | NC | ND |
10 | 61 | 59 | 60 | 60 | NDc | 34 | 27 | 28 | 24 | ND |
14 | 49 | 40 | 57 | 55 | 10* | 21 | 39 | 31 | 47 | 9§* |
21 | ND | ND | ND | ND | 48* | ND | ND | ND | ND | ND |
28 | 42* | 49* | 40* | 19* | 17* | 44§* | 41§* | 20§* | ND | 23 §* |
214 | ND | ND | ND | ND | 7* | ND | ND | ND | ND | ND |
365 | 3* | ND | ND | ND | 5* | ND | ND | ND | ND | ND |
B. burgdorferi B31 Clones 5A4 or 5A3 (asterisks) were injected subcutaneously 105/mouse at the base of the tail. Groups of 4 to 6 mice were sacrificed on the indicated days post infection.
Cultures from the tissue sites (urinary bladder, heart, tibiotarsal joint, ear pinnae and skin punch biopsies) were acquired under aseptic conditions and clones obtained by subsurface colony formation in agar plates.
SCID mice were either C3H/HeN SCID or CB-17 SCID (§).
NC = no positive cultures obtained.
ND = cultures not done.