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Layout of microRNA arrays. There were 16 print-tips, and blocks were laid out on a 4 by 4 grid. The majority of spots do not represent human microRNAs. Of the ones that do, each are spotted in duplicate, so there are ∼450 unique microRNAs in total. Included among the nonhuman microRNAs are 15 spike-in controls, also spotted in duplicate.
