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. 2008 Dec 4;37(2):516–525. doi: 10.1093/nar/gkn964

Figure 1.

Figure 1.

DDB directly binds to XPA through DDB2 subunit. Purified DDB heterodimer (A) or each DDB subunit (B) was incubated with GST alone (lane 2) or GST-XPA (lane 3) coupled to glutathione–sepharose 4B beads. The bound proteins were separated on a SDS–polyacrylamide gel and analyzed by western blotting with anti-Flag antibody for detecting DDB1 and DDB2. (C) Purified (His)6-XPA protein was incubated with MBP alone (lane 2) or MBP-DDB2 (lane 3) coupled to amylose beads. The bound proteins were analyzed by western blotting with anti-His antibody.