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Domain mapping of XPA responsible for the binding to DDB2. (A) Schematic diagram of various XPA deletion mutants and summary of pull-down experiments with these mutants. (B) The lysates from insect cells overproducing DDB2 were incubated with GST alone (lane 1) or various GST-XPA derivatives (lanes 2–7) coupled to glutathione–sepharose 4B beads. The bound proteins were analyzed by western blotting with anti-Flag antibody.
