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. 2008 Dec 4;37(2):516–525. doi: 10.1093/nar/gkn964

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Effect of R207G mutation on XPA accumulation at damaged DNA sites after local UV irradiation. (A) myc-tagged XPA, wild-type or R207G mutant, was transiently expressed in XP2YOSV (upper panel) or XP2YOSV/F-DDB2 cells (middle and lower panels). Cells were irradiated with 20 J/m² of UV light through an isopore polycarbonate membrane filter. After 30 min incubation, cells were fixed and co-stained with anti-myc antibody (XPA) and TDM-2 antibody (CPD). (B) myc-tagged XPA, wild-type or R207G mutant, and 3xFlag-tagged DDB2 were transiently co-expressed in XP2YOSV cells. Following micropore UV irradiation, cells were fixed and co-stained with anti-myc antibody (XPA) and anti-Flag antibody (DDB2).