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. 2008 Dec 4;37(2):516–525. doi: 10.1093/nar/gkn964

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Effect of R207G mutation on the enhancement of CPD repair by ectopic expression of DDB2 in SV40-transformed human cells. (A) Cell lysates were prepared from WI38VA13/3xF-DDB2 and XP129/3xF-DDB2 cells and analyzed by immunoblotting with anti-Flag and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibodies. (B) WI38VA13/3xF-DDB2, XP129/3xF-DDB2 and their parental cells were irradiated with 10 J/m² of UV-C light and incubated for the indicated periods. Genomic DNAs were purified and used for the determination of CPD content by an enzyme-linked immunosorbent assay. Each point represents the mean of three independent experiments and error bars indicate the SD.