Table 2.

Self-cleavage rate constants of the wild-type CChMVd (−) and (+) hammerheads and some single and double mutants

Variant	kobs (min−1)
(−)	Wild-type	2.58 ± 0.11
	IIa	2.36 ± 0.28
	L1.U6Aa	0.05 ± 0.01
	L1.U6Ga	0.16 ± 0.02
	L1.U6Ca	0.38 ± 0.06
	L1.U6Δa	0.34 ± 0.05
	L1.A4Ga	2.25 ± 0.41
	L1.U1Aa	0.13 ± 0.02
	IL2.A8Ua	0.066 ± 0.002
	L1.U1A/IL2.A8Ua	1.09 ± 0.07
	L1.G2Aa	0.90 ± 0.19
	IL2.C7Ua	0.59 ± 0.01
	L1.G2A/IL2.C7Ua	0.84 ± 0.04
(+)	Wild-type	3.33 ± 0.80
	L1.U6A	0.049 ± 0.014
	L1.U1C	0.91 ± 0.08
	L2.A6G	2.76 ± 0.72
	L1.U1C/L2.A6G	0.042 ± 0.003
	L1.G2A	0.79 ± 0.09
	L2.C5U	2.18 ± 0.16
	L1.G2A/L2.C5U	0.28 ± 0.07

Single mutants with k_obs ≤ 0.1 min⁻¹ are shown in bold.

^aSmaller version of the (−) hammerhead in which the domain II helix was shortened (Figure 1A).