Table 1.
Binding of TEL and TEL*-based sequences to Cdc13(DBD)
| Oligonucleotide | Sequence | Kd,appa (nM) | Relative Kdb |
|---|---|---|---|
| TEL | GTGTGGGTGTG | 2.8 ± 0.6 | − |
| TEL* | GTGTcTGTcTG | 620 ± 120 | 220 |
| TEL*-a10 | GTGTcTGTcTGaaaaaaaaaa | 380 ± 57 | 140 |
| TEL*-GG | GTGTcTGTcTGGG | 120 ± 19 | 43 |
| TEL*-GGG | GTGTcTGTcTGGGG | 33 ± 5.7 | 12 |
| TEL*-GTGGG | GTGTcTGTcTGGTGGG | 11 ± 1.3 | 4 |
| TEL*-GTGTGGG | GTGTcTGTcTGGTGTGGG | 24 ± 4.3 | 8.6 |
| TEL*-GTGTGTGGG | GTGTcTGTcTGGTGTGTGGG | 13 ± 1.8 | 4.6 |
aBinding experiments were performed in 750 mM KCl to attenuate the picomolar affinity of Cdc13(DBD) for TEL sequence to the nanomolar range. Kd values shown are averages of at least three independent apparent Kd measurements ± standard error.
bKd values presented are relative to the Kd of the TEL oligonucleotide.