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. 2008 Dec 2;37(2):431–440. doi: 10.1093/nar/gkn955

Figure 3.

Figure 3.

Promoter occupancy by WT1 and BASP1 in differentiating MPC5 cells. (A) ChIP was performed with undifferentiated MPC5 cells (0) or MPC5 cells that had differentiated for 5 days (5). The antibodies used in the ChIP were rabbit anti-GAL4 antibodies (αCtrl), anti-acetylated histone H3 (αAcH3), rabbit anti-WT1 antibodies (αWT1), rabbit anti-BASP1 antibodies (αBASP1r) and sheep anti-BASP1 antibodies (αBASP1s). The resultant samples were subject to amplification by PCR using primers directed to the mouse PDGFA promoter (top) or CSF1 promoter (bottom). I is 1/50 of the input chromatin in each immunoprecipitation. A diagram of each promoter region is shown at right, with the positions of the WT1 DNA-binding sites and forward/reverse primer regions indicated. (B) ChIP was performed as in part A except that the Bak (top) and c-myc (bottom) promoters were analysed. (C) As in (A) except that the analysis was performed with undifferentiated MPC5 cells (0) and cells that had been allowed to differentiate for 3 (3) and 5 (5) days. The bottom panel is amplification of an internal region of the podocalyxin gene (Int.) from ChIP analysis of undifferentiated MPC5 cells.