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. 2008 Nov 28;37(2):322–335. doi: 10.1093/nar/gkn940

Figure 4.

Figure 4.

Cell-cycle changes after overexpression of IRF2BP2. Cell-cycle analysis of U2OS cells transfected either with an empty vector or with an IRF2BP2-expression construct. Transfection efficiency was monitored by GFP and BrdU-staining was performed for analysis. (A) The transfected cells were left untreated prior to harvest. (B) After transfection the cells were treated with 2.5 nM Act.D for 12 h prior to cell-cycle analysis. (C) Treatment of the transfected cells with 1 nM of doxorubicin for 18 h. (D) Treatment of the transfected cells with 1 nM of doxorubicin for 24 h. (E) Determination of levels of apoptosis after transfection of cells with IRF2BP2 or empty vector. Sub-G1 population was measured after treatment of cells by propidium iodide staining. All SD result from three biological replicas, each performed in duplicate. Statistical significance was determined by Student's t-test (*P < 0.05; **P < 0.01; ***P < 0.001).