Figure 5.

Electrophoretic mobility-shift assays of transpososomes formed with Δ369 EK/MA transposase and H-NS. (A) H-NS mobility-shifts with transpososomes formed with either HA transposase or Δ369 EK/MA transposase. (B) Mobility shift with Δ369 EK/MA and either wild-type (WT) or P116S H-NS. H-NS-transpososomes in (A) and (B) were assembled under the ‘ME+T’ase+H-NS’ regimen, and the substrate is the same as in Figure 2B. Species are labeled as in Figure 2. (MC) monomer complex; (H-NS-MC) H-NS monomer complex; (H-NS-MC het) a heterogeneous mixture of H-NS monomer complexes.