Table 1.

In vivo transposition of mini-Tn5-Tet^R catalyzed in trans by HA transposase in hns⁺/hns⁻ strains

Transposase source	Strain	Transposition frequencya	Normalized frequencyb
pDH390	NK5830	8.1 (±0.0200) × 10−2	1.00
pDH390	NK5830hns::KanR	2.6 (±0.0097) × 10−2	0.32

^aRelative transposition frequencies were calculated by dividing the number of Sm^RTet^R colonies (transposition events) by the number of Sm^R colonies (total exconjugants) obtained per 0.1 ml of mating mix. Transposition frequencies represent an average value obtained from three independent experiments wherein matings with at least eight different donor transformants were carried out in each experiment.

^bTransposition frequencies were normalized to the level of transposition catalyzed by the HA transposase in the hns⁺ strain.