Table I.
Kinetic and dissociation constants for the interaction ofC1q or its GR and PS and its derivativesa
| Soluble Analyte/Immobilized Ligand | Kon(M-1 × s-1) | Koff(s-1) | kD(M) |
|---|---|---|---|
| C1q/PSb | 2.9 × 104 | 2.1 × 10-3 | 7 × 10-8 |
| C1q GR/PSb | 3.5 × 103 | 1.8 × 10-3 | 5 × 10-7 |
| 06:0 PS/C1qc | 13.6 | 2.4 × 10-3 | 1.7 × 10-4 |
| C1q/phosphoserinec | 8.4 × 104 | 3.1 × 10-3 | 3.7 × 10-8 |
a
Interactions were measured on either HPAb or CM5c sensorchips, as described in Materials and Methods. The data shown correspond to a representative series of binding studies performed on the same sensor chip. In each case, similar results were reproduced from at least three independent experiments, using different phospholipid and protein preparations, and different sensor chips.