TABLE 3.
Antigenic characterization of viruses by HI assay with anti-H9 monoclonal antibodies and polyclonal chicken antisera
| Polyclonal chicken antiseraa | Anti-H9 monoclonal antibodies | |||||
|---|---|---|---|---|---|---|
| H9N2Virus | aA/ty/Israel/90710/00 | a A/ty/Israel/1567/04 | aA/Qa/HK/G1/97 | aA/Ck/HK/G9/97 | aA/Dk/HK/Y280/97 | |
| G1-26 | G9-6 | 18G4 | 2F4 | |||
| A/ty/Israel/90710/00 | 40 | 160 | 200 | 200 | <b | < |
| A/ty/Israel/965/02 | 80 | 80 | 100 | 800 | < | < |
| A/ty/Israel/1567/04 | 320 | 640 | 12800 | 200 | 100 | 1600 |
| A/Qa/HK/G1/97 | 80 | 320 | 6400 | 3200 | < | < |
| A/Ch/HK/G9/97 | 160 | 1280 | 3200 | 6400 | 3200 | 3200 |
| A/Dk/HK/Y280/97 | 80 | 320 | 800 | 6400 | 25600 | 25600 |
a
Polyclonal anti-H9 antisera obtained at day 28 p.i. with H9N2 virus. To remove non-specific inhibitors of agglutination, serum was incubated overnight with receptor-destroying enzyme and heated for 30 min at 56°C. The initial dilution was 1:40.
b
< Indicates a titer less than 1:100 (the initial dilution of the antibodies).