Table 1.
Relative gene primers sequence
| Target gene | Primer sequence | Genebank No. | Production length (bp) | |
|---|---|---|---|---|
| 18S | Forward primer | 5′-GCGGTTCTATTTTGTTGG-3′ | M11188.1 | 120 |
| Reverse primer | 5′-AATGCTTTCGCTCTGGTC-3′ | |||
| ChAT | Forward primer | 5′GATGTTCTGCTGTTATGGACCCG-3′ | XM001061520.1 | 232 |
| Reverse primer | 5′-AGTCAAGATTGCTTGGCTTGGTT-3′ | |||
| VAChT | Forward primer | 5′GTGCGAGGACGACTACAACTATT-3′ | U09211.1 | 101 |
| Reverse primer | 5′-TCTTGCAGAATGACCATCTTGAC-3′ | |||
| CHT1 | Forward primer | 5′-ATGGAACTTGCTGATAGGATGGA-3′ | NM053521.1 | 121 |
| Reverse primer | 5′ATGATTCTCCCGATTGTTCTACAG-3′ |