Table 2.

TILLING analysis for MtPHY1 and the putative M. truncatula orthologue of MsTI from Medicago scutellata

Gene	GenBank	Primer pair	No. of mutations	Type of mutation
MtPHY1	AY878355	5'-AACAATGCCGGTTTCAGGTC 5'-TTGAAGGCGCAGAAACCTCT	8	C → T (4) G → A (4)
Trypsin inhibitor	AC135311	5'-TGTTTGTTTCGTCTGGAGCA 5'-GTGGACGGTCTCCCAGACTC	7	C → T (2) G → A (5)