Figure 1. Generation of Prom1^C-L mice and reporter analysis.

(a) Top to bottom: CreERT2-IRES-nuclear(n)LacZ-PGK-Neo was cloned into the ATG site of Prom1. pProm1 was subject to homologous recombination with wild type Prom1 to generate the Prom1^C-L targeted allele. PGK-Neo was excised by flip-recombination. p=Southern blot probe; A=ApaL1 sites; FRT=flip recombinase targets. Southern blot of mouse tissues heterozygous for the targeted allele (lane 1) and homozygous for the wild-type allele (lane 2). (b) Expression of nLacZ from the Prom1^C-L allele in embryonic tissues. NT, neural tube; R, rib; L, limb; RT, renal tubule. Scale bars=200μm. (c) Prom1 and nLacZ expression in adult mouse (3 month) tissues. β-galactosidase staining (left panel), Prom1 protein (middle panel, immunofluorescence) and markers (right panel) of neural stem and progenitor cells (Nestin), Clara cells (Clara cell specific protein [CCSP]), pancreatic ductal cells (Dolichos Biflorus Agglutinin [DBA]-fluorescein), proximal renal tubular endothelial cells (Lotus Tetragonolobus Lectin [LTL]-fluorescein), and photoreceptors (rhodopsin). Middle and right panels are adjacent sections of the boxed areas in left panels. V, ventricle; B, bronchiole; * vessel at bronchioalveolar junction; PD, pancreatic duct; RT, renal tubule; PR, photoreceptor. All scale bars=50μm.