Figure 6. Freezing responses of subjects after pre-exposure to the CS paired with playback of object distress vocalizations.
(a) Subjects were exposed to objects during conditioning, but the CS-UCS was not presented to objects (N = 10 subjects/genotype; age = 5–7 wks). (b) Subjects were exposed to objects receiving the paired CS-UCS during conditioning (N = 8 subjects/genotype; age = 5–6 wks). (c) Subjects were not exposed to objects, but received the CS forward paired with the playback of object distress vocalizations during conditioning (N = 16 subjects/genotype; age = 5–7 wks). Asterisks represent a significant (P<0.05) difference between subjects from the BALB and B6 strains. All data were scored in duplicate by 2 independent raters and are presented as the mean±s.e.m. Inter-rater reliability, rp = 0.96, d.f. = 659.