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. 2009 Feb 6;4(2):e4392. doi: 10.1371/journal.pone.0004392

Figure 2. Efficiency of primary NS formation in the presence of different growth factors and rate of expansion of fetal vs. adult NS.

Figure 2

A, Comparison of the number of NS formed in either fetal or adult hypothalamic cultures in the presence of FGF-2 after 7DIV. B, Comparison of the numbers of primary NS formed in fetal hypothalamic cultures in the presence of either EGF, FGF-2 or EGF plus FGF-2 after 7DIV. C, Comparison of the numbers of primary NS formed in adult hypothalamic cultures in the presence of either EGF, FGF-2 or EGF plus FGF-2 after 7DIV. The insets underneat this graph show the expression of the growth factor receptors for EGF (EGFR), CNTF (CNTFR/gp130/LIFR ) and BDNF (TrkB), as detected by RT-PCR in the RNA prepared from adult hypothalami. D, Comparison of fetal vs. adult NS rate of expansion upon serial passages, all cultures were kept in the presence of FGF-2 plus EGF and counted after 7DIV, respectively before passage 2 (secondary NS), 4 or 6. Values are average number of NS±SEM (n = 3 or 4 independent experiments).