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. Author manuscript; available in PMC: 2009 Sep 26.
Published in final edited form as: Circ Res. 2008 Aug 21;103(7):702–709. doi: 10.1161/CIRCRESAHA.108.175489

Figure 1.

Figure 1

Pbx proteins are widely present in tissues relevant to heart development. A through C, Immunohistochemical analysis of Pbx proteins at E9.5. Pbx1b (A) is present in neural crest cells (arrow), mesenchymal cells of the branchial arch region (asterisk), endocardial cells (arrowhead), and myocardial cells (double arrows) of the cardiac OFT. Pbx2 (B) and Pbx3 (C) display similar expression patterns at E9.5. D through F, Presence of Pbx proteins in the cardiac OFT at E12.5. Pbx1b (D) is present in endocardial (arrowhead) and mesenchymal cells (asterisk) of the endocardial cushions of the OFT. Pbx1b is downregulated in myocardial cells (double arrow) at E12.5. Pbx1b is present in the endothelial and vascular smooth muscle cells of the aorta (Ao) and main pulmonary artery (MPA). Pbx2 (E) is present in endocardial (arrowhead), myocardial (double arrow), and mesenchymal cells (asterisk) of the OFT. Pbx2 is also present in endothelial and vascular smooth muscle cells of the aorta and main pulmonary artery. Pbx3a (F) is present in endocardial (arrowhead) and mesenchymal cells (asterisk) of the cushion but downregulated in myocardial cells (double arrow). Pbx3a is present in endothelial but not vascular smooth muscle cells of the great arteries.