Figure 7.
(A) Terminal deoxynucleotidyltransferase dUTP nick end labeling (TUNEL)-positive cells (green) in saline- and A12-treated mouse lungs on Days 14 and 28 after bleomycin injury. Nuclei are counterstained with 4′,6-diamidino-2-phenylindole (DAPI). Original magnification: ×20. (B) Quantification of TUNEL IHC by determining the number of TUNEL-positive cells per total number of cells. Shown is the average and SD of two mice per condition, with at least 500 cells analyzed per condition. Data were analyzed by two-way analysis of variance with Tukey's honestly significant difference (HSD) post-hoc test. (C) Increased apoptosis of primary mouse lung fibroblasts treated with insulin-like growth factor-I receptor (IGF-IR) antibody. Mouse lung fibroblasts were serum starved overnight and then incubated with the indicated concentration of IGF-IR antibody A12. Apoptosis was measured with Cell Death Detection ELISAPLUS (Roche Applied Science). All experiments were done in triplicate and repeated at least twice. The apoptosis index is defined as follows: the ratio of the optical density at 405 nm (OD405 nm) of the experimental condition to that of the control condition (medium alone). *P < 0.05 compared with the control group.