Figure 9.

Insulin-like growth factor-I (IGF-I)–induced migration of fibroblasts. Mouse lung fibroblasts were plated on FluoroBlok transwell filters overnight, and then IGF-I (100 ng/ml), serum-free medium (negative control), or 10% serum (positive control) was added to the lower chamber. Some cells were preincubated with the blocking antibody to IGF-I receptor (A12, 40 μg/ml) before the addition of IGF-I. Cells were allowed to migrate through the membrane for 4 hours at 37°C. Migrated cells were counted by fluorescence microscopy. Each experiment was done in triplicate and repeated at least three times. Average cell counts and SD are presented.