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. 2008 Oct 17;179(2):138–150. doi: 10.1164/rccm.200804-535OC

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Figure 8. — Suppression of antioxidant response element (ARE)-responsive antioxidant/detoxifying enzyme induction by RSV in Nrf2^−/− mice. (A) Expression of pulmonary ARE-driven antioxidant genes determined by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Representative digitized image from semi-quantitative RT-PCR demonstrates increased Nrf2 and ARE-driven antioxidant mRNA expression. Quantitative RT-PCR assessed the relative ratio of each cDNA levels over Nrf2^+/+-vehicle control after normalization to corresponding 18s rRNA levels, and data presented in graphs are the normalized group mean ± SEM (n = 3/group). *Significantly different from genotype-matched vehicle control mice (P < 0.05). ⁺Significantly different from exposure-matched Nrf2^+/+ mice (P < 0.05). (B) Western blot analyses of lung total proteins from Nrf2^+/+ and Nrf2^−/− mice determined Nrf2-dependent protein expression of pulmonary antioxidant/detoxifying enzymes after RSV infection. Actin levels are shown as internal controls. Representative digitized bands from multiple analyses (n = 3/group) are presented.

Figure 8. — Suppression of antioxidant response element (ARE)-responsive antioxidant/detoxifying enzyme induction by RSV in Nrf2^−/− mice. (A) Expression of pulmonary ARE-driven antioxidant genes determined by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Representative digitized image from semi-quantitative RT-PCR demonstrates increased Nrf2 and ARE-driven antioxidant mRNA expression. Quantitative RT-PCR assessed the relative ratio of each cDNA levels over Nrf2^+/+-vehicle control after normalization to corresponding 18s rRNA levels, and data presented in graphs are the normalized group mean ± SEM (n = 3/group). *Significantly different from genotype-matched vehicle control mice (P < 0.05). ⁺Significantly different from exposure-matched Nrf2^+/+ mice (P < 0.05). (B) Western blot analyses of lung total proteins from Nrf2^+/+ and Nrf2^−/− mice determined Nrf2-dependent protein expression of pulmonary antioxidant/detoxifying enzymes after RSV infection. Actin levels are shown as internal controls. Representative digitized bands from multiple analyses (n = 3/group) are presented.