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. 2008 Dec 4;28(1):21–33. doi: 10.1038/emboj.2008.238

Figure 1.

Figure 1

PTEN is not induced in tissues of the EGR1−/− or ARF−/− mouse models. (A) Analysis of PTEN mRNA levels by qRT–PCR in six tissues from 8-week-old, healthy EGR1−/− and wild-type mice that were γ-irradiated with 5 Gy and killed 2.5 h later. (B) A similar qRT–PCR analysis for PTEN mRNA in four tissues: liver, kidney, lung, and heart (and also spleen, data not shown) from ARF−/− 129sv/BL6 mice and wild-type mice of the same strain that were γ-irradiated with5 Gy and killed 0, 1, 3, or 7 h later. (C) Western blot analysis for PTEN, Akt1, and phospho-Akt1 in two tissues liver and lung from the same mice in (B). Luciferase activity measured 24 h after transfection into 293T cells (D) ARF−/− and ARF+/+ MEFs (E) with the indicated DNA constructs and siRNAs. The PTEN-luciferase promoter construct was described earlier (Virolle et al, 2001) and the same reporter Δ5-PTEN-luc lacking most EGR1-binding sites is shown in Supplementary Figure S2. The data represent the mean and triplicate determinations±s.d. (*P<0.05, **P<0.01, ***P<0.001).