Figure 6.

Inhibiting p38 MAP kinase phosphorylation does not alter C/EBP levels. (A) Transcription factor binding to C/EBP consensus sequence, ICAM-1 gene HFRE sequence from −200 to −135, or control CRE sequence was assessed using EMSAs with nuclear extracts from hTBE cell monolayers that were left untreated or were pretreated with a p38 inhibitor at the indicated concentrations, and then incubated with H. influenzae for 4 hours. (B) C/EBP family nuclear protein levels were assessed using immunoblot analysis of nuclear protein extracts from hTBE cell monolayers that were left untreated or were pretreated with a p38 inhibitor at the indicated concentrations. Cells were then incubated without or with H. influenzae for 4 hours. Results in both panels are representative of three experiments.