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CD4+ and CD8+ cells are not responsive to DEX after treatment with IL-2/IL-4 for 48 hours. CD4+ and CD8+ T cells were purified by negative selection from PBMC cultures. GCR was measured in cellular nuclear extracts from media- (A) versus IL-2/IL-4 (B)–treated cells ± DEX (1 h) based on its interaction with glucocorticoid response elements (GRE) consensus motif immobilized to the plate (TransAM GR transcription factor assay) (n = 4). Data are presented as means ± SEM.
