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IL-2/IL-4 combination inhibits mitogen-induced mitogen-activated protein kinase (MAPK) phosphatase (MKP)-1 mRNA induction by DEX. At the end of the 48-hour incubation with cytokines, the cells were treated with 10−6 M DEX for an additional 4 hours, or remained untreated. MKP-1 mRNA induction by DEX in cell isolates as compared with medium-treated cells was analyzed by real-time PCR.
