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ATPase activity of Type III REs. (A) Kinetics of ATP hydrolysis (per DNA) for EcoPI and EcoP15I measured for capped and uncapped HtH substrates at 1 mM ATP. The ATPase rates indicated are for capped and uncapped (in brackets) substrates. (B) Average number of ATP molecules required to cleave a DNA molecule obtained by dividing the ATPase rate by the cleavage rate measured under the same conditions (Fig. S4).
