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. 2009 Jan 30;106(6):1886–1891. doi: 10.1073/pnas.0812945106

Fig. 4.

Fig. 4.

Detailed analysis of t(4;11)(q32;q21). (A) Local genomic features of chromosomes involved. The interchromosome translocation between chromosomes 11q21 and 4q32 truncates MRE11A at its DNA binding domain. Chimeric cDNAs span exon 9, 10 and 11 (brown bars) of MRE11A and intergenic sequences on chromosome 4 as shown by thick red arrows. A 9- to 10-kb genomic fragment containing the break junction was amplified with primers on chromosome 11 and chromosome 4 as shown in light blue arrows. Consensus splice acceptor sequences used for transcription of the chimeric cDNA on chromosome 4 are shown. LINE repeats are shown in shaded gray bars. The transcription orientation of MRE11A gene is marked by a black arrow. An in-frame stop codon in the chimeric 454 cDNA is marked by an asterisk. Coverage of 454 reads (shown by shaded pink areas) mapped to the 9- to 10-kb fragment was determined from an assembly output graph from CLC Bio. Coverage of the genome at the break junction is between 500× and 3,200×. (B) Final fine assembly of the break junction of t(4;11)(q32;q21) by mapping and assembly of 454 sequences on the 9- to 10-kb genomic fragment.