Fig. 3.
Allogeneic T cell proliferation and cytokine secretion by dendritic cells (DCs) matured with different combinations of maturation stimuli. (a) Capacity of mature DCs (mDCs) generated with different combinations of maturation stimuli to induce allogeneic T cell proliferation. Immature DCs were stimulated with the indicated maturation stimuli for 48 h, and the mDCs were used to stimulate proliferation of allogeneic peripheral blood mononuclear cells (2 × 105 cells/well) at different stimulator : responder ratios. Data from the stimulator : responder ratio at 1:10 are presented as mean counts of incorporated [3H]-thymidine. Results shown represent the mean ± standard error of the mean (s.e.m.) of four independent experiments. *Increased or decreased cell proliferation with respect to control (DCs matured with cytokine cocktail) is statistically significant (P < 0·05). (b) IL-10 and (c) IL-12p70 were measured in culture supernatants 48 h later. Results are expressed as mean ± s.e.m. of six independent experiments. *Increased cytokine production with respect to control (DCs matured with cytokine cocktail) is statistically significant (P < 0·05).