Fig. 4.

Intracellular cytokine expression of dendritic cell (DC)-activated T cells. Immature DCs, DCs matured with either cytokine cocktail, polyinosinic : polycytidylic acid [poly(I:C)] (20 µg/ml) alone or a combination of poly(I:C) (20 µg/ml) with tumour necrosis factor-α (10 ng/ml), peptidoglycan (10 µg/ml) and CD40L (1 µg/ml) were co-cultured with allogeneic CD4⁺ T cells. After 7 days of co-culture, T cells were restimulated with phorbol 12-myristate 13-acetate and ionomycin for 8 h. Brefeldin A was added to the culture during the last 6 h before staining to prevent cytokine release. Quadrants were set according to the fluorescence intensities of isotype-matched control antibodies with irrelevant specificity. Results from one representative of three independent experiments are shown.