Fig. 5.
Chemotactic response of dendritic cells (DCs) in response to major inflammatory protein (MIP)-3β and interleukin (IL)-8. DCs (1 × 105) were seeded in the upper compartment of Transwell plates and incubated for 2 h at 37°C in the presence or absence of MIP-3β (10 ng/ml) or IL-8 (50 ng/ml) added to the lower compartment. DC counts in the lower chamber wells were measured by flow cytometry. Chemotaxis in the absence of any chemokines (medium) was the negative control. Results from one representative of three independent experiments are shown.